Assessment of biological tests for Pneumocystis Pneumonia (Pneumocystis jirovecii infections) diagnosis - INAHTA Brief

The aim of this work is to evaluate the accuracy of biological techniques for the diagnosis of Pneumocystis Pneumonia (bilateral pneumonitis caused by human-specific fungus Pneumocystis jirovecii) in immunosuppressed individuals. It focuses on direct identification of specific fungal organisms by microscopic methods (immunofluorescence -IF, staining methods) and Pneumocystis-specific DNA detection by polymerase chain reaction (PCR) in respiratory fluid samples. In addition, it evaluates the dosage of the soluble antigen β -(1-3)- D - glucane (BG) in blood.